Doubled haploids (DH) have become a helpful tool for both basic and applied research. The primary, though not exclusive, regular utilization of double haploids by breeding industries is to produce pure lines for seed production among various crops. After many decades of finding of haploid inducer lines in maize and to develop haploid plants from its pollen precursors anther culture was used, with other biotechnological advance tools have helped to develop various techniques for DH production. It is now feasible to make haploids and DH in various species, as there are multiple methods to test if one does not work effectively. This chapter provides an overview of the recent approaches to producing haploids and DHs, by using in vitro, in vivo or using both techniques for embryo formation.
Haploid, anther, wide hybridisation, double haploid (DH), auxin
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