Next-generation sequencing (NGS) has revolutionized crop improvement by providing high-throughput, cost-effective and rapid genotyping technologies. The NGS technology has revolutionized genomic research, enabling the sequencing of thousands of plant genomes. This has facilitated the identification of millions of new markers and agronomically important genes for crop improvement. This technology has significantly advanced our understanding of genetic diversity, gene function, and evolutionary relationships among organisms. In the field of agriculture, NGS has emerged as a powerful tool for crop improvement, offering unusual opportunities to enhance crop yield, quality, and sustainability. This chapter aims to give an overview of different generations of sequencing and its various applications in crop improvement.
ABI/SOLiD, Illumina/Solexa, Ion torrent, Next-generation sequencing, Pyrosequencing
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